Device

Part:BBa_K300014

Designed by: Lorenzo Pasotti, Susanna Zucca, Nicolo' Politi   Group: iGEM10_UNIPV-Pavia   (2010-10-16)

Self-inducible device with J23110


This is a self-inducible device library member. It is a specialized version of BBa_K300010 PoPS-based device. E. coli bearing this part are expected to accumulate HSL in the growth medium with a HSL synthesis rate dependant on the strength of the promoter that regulates luxI BBa_C0061.

Quantitative information about this part and the whole library are availavble in BBa_K300010 Registry page.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 701
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1856


[edit]
Categories
Parameters
None